Autoradiography is one of the imaging techniques that employ radioactive sources. These radioactive substances are present in the exposed sample. This technique can be performed in the lab as well in the living systems. When performed in a laboratory, the components of the cell i.e., nucleic acids, lipids and proteins are isolated and are then labelled with specified radioisotopes.
On the other hand, in vivo autoradiography employs either the oral intake of the radioisotopes that are coupled with radioactive tracers or they may be injected. The distribution of the radiations is then determined in the tissues. The most widely used radioisotopes are carbon-14, sulfur-35, phosphorus-32 etc.
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