Q: What is the major difference between isocratic elution and gradient elution?
What is the major difference between isocratic elution and gradient elution? For what types of compounds are these two elution methods most suited?
See AnswerQ: Why are fragments often produced with electron ionization?
Why are fragments often produced with electron ionization?
See AnswerQ: Describe two types of pumps used in HPLC. What are the
Describe two types of pumps used in HPLC. What are the advantages and disadvantages of each?
See AnswerQ: Describe the differences between single-column and suppressor-column ion
Describe the differences between single-column and suppressor-column ion chromatography.
See AnswerQ: Mass spectrometry is an extremely versatile detection system for gas chromatography.
Mass spectrometry is an extremely versatile detection system for gas chromatography. Describe the major reasons why it is more difficult to combine HPLC with mass spectrometry than it is to combine GC...
See AnswerQ: Which of the GC detectors in Table 30-1 are suitable
Which of the GC detectors in Table 30-1 are suitable for HPLC? Why are some of these unsuitable for HPLC? Table 30-1:
See AnswerQ: The ideal detector for GC is described in Section 30A-4
The ideal detector for GC is described in Section 30A-4. Which of the eight characteristics of an ideal GC detector are applicable to HPLC detectors? What additional characteristics would be added to...
See AnswerQ: Although temperature does not have nearly the effect on HPLC separations that
Although temperature does not have nearly the effect on HPLC separations that it has on GC separations, it nonetheless can play an important role. Discuss how and why temperature might or might not in...
See AnswerQ: Two components in an HPLC separation have retention times that differ by
Two components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 minutes, and the peak widths are approximately equal. The void time, tM, was 63 s. Use a sp...
See AnswerQ: An HPLC method was developed for the separation and determination of ibuprofen
An HPLC method was developed for the separation and determination of ibuprofen in rat plasma samples as part of a study of the time course of the drug in laboratory animals. Several standards were chr...
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