Q: Let’s suppose a researcher was interested in the effects of mutations on
Let’s suppose a researcher was interested in the effects of mutations on the expression of a protein-encoding gene that encodes a polypeptide that is 472 amino acids in length. This polypeptide is exp...
See AnswerQ: What environmental conditions favor a switch to the lytic cycle?
What environmental conditions favor a switch to the lytic cycle?
See AnswerQ: Explain the basis for using an antibody as a probe in a
Explain the basis for using an antibody as a probe in a Western blotting experiment.
See AnswerQ: A cloned gene fragment contains a regulatory element that is recognized by
A cloned gene fragment contains a regulatory element that is recognized by a regulatory transcription factor. Previous experiments have shown that the presence of a hormone results in transcriptional...
See AnswerQ: Describe the rationale behind the electrophoretic mobility shift assay.
Describe the rationale behind the electrophoretic mobility shift assay.
See AnswerQ: Certain hormones, such as epinephrine, can increase the levels of
Certain hormones, such as epinephrine, can increase the levels of cAMP within cells. Let’s suppose you pretreat cells with or without epinephrine and then prepare a cell extract that contains the CREB...
See AnswerQ: An electrophoretic mobility shift assay can be used to study the binding
An electrophoretic mobility shift assay can be used to study the binding of proteins to a segment of DNA. In the experiment shown here, an EMSA was used to examine the requirements for the binding of...
See AnswerQ: As described in Chapter 15 (Figures 15.7 and 15
As described in Chapter 15 (Figures 15.7 and 15.8), certain regulatory transcription factors bind to DNA and activate RNA polymerase II. When glucocorticoid binds to the glucocorticoid receptor (a reg...
See AnswerQ: In the technique of DNase I footprinting, the binding of a
In the technique of DNase I footprinting, the binding of a protein to a region of DNA protects that region from digestion by DNase I by blocking the ability of DNase I to gain access to the DNA. In th...
See AnswerQ: Explain the rationale behind a DNase I footprinting experiment.
Explain the rationale behind a DNase I footprinting experiment.
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