Electrophoretic mobility shift assay is a technique that is used to study the interaction of proteins with DNA and RNA fragments. This method enables us to determine whether a protein or a mixture of proteins has an ability to get bound to a given DNA or RNA molecule or not. It is also used as a measure that indicates the number of protein molecules participating in the binding complex.
In this method, the mobility of protein is retarded when it gets bound to the DNA or RNA fragment. It is performed in the labs when the processes like DNA replication, transcription, DNA repair, mRNA splicing etc. are studied.
An electrophoretic mobility shift assay (EMSA) can be used to
As described in Chapter 21 and in experimental question E3, an
A cloned gene fragment contains a regulatory element that is recognized by
Certain hormones, such as epinephrine, can increase the levels of
An electrophoretic mobility shift assay can be used to study the binding
As described in Chapter 21, an electrophoretic mobility shift assay (
