Definition of Electrophoretic Mobility Shift Assay

Electrophoretic mobility shift assay is a technique that is used to study the interaction of proteins with DNA and RNA fragments. This method enables us to determine whether a protein or a mixture of proteins has an ability to get bound to a given DNA or RNA molecule or not. It is also used as a measure that indicates the number of protein molecules participating in the binding complex.


In this method, the mobility of protein is retarded when it gets bound to the DNA or RNA fragment. It is performed in the labs when the processes like DNA replication, transcription, DNA repair, mRNA splicing etc. are studied.


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