A spectrophotometer is a laboratory instrument which is used for the quantitative measurement of the transmitted visible light, ultraviolet light and infrared light. The spectrophotometers are classified into two classes i.e., single beam and dual beam.
The single beam spectrophotometers are responsible for measuring the absolute intensity of light. On the other hand, the double beam spectrophotometers tend to measure the proportion of light intensities on the reference standard and sample.
The spectrophotometers are commonly employed in the fields of physics, molecular biology, biochemistry, chemistry etc. The concentration of the proteins, nucleic acids (DNA and RNA), the growth of the bacterial culture and the enzymatic reactions can be determined by the technique of spectrophotometry.
Testing patient blood samples for HIV antibodies, a spectrophotometer determines the
Hashish is a concentrated resin secreted by the Cannabis plant.
The following data were taken from a diode-array spectrophotometer in
A typical simple infrared spectrophotometer covers a wavelength range from 3 to
A solution with a “true” absorbance [A = -
