Q: Figure 11.4b shows an autoradiograph of a replicating bacterial chromosome
Figure 11.4b shows an autoradiograph of a replicating bacterial chromosome. If you analyzed many replicating chromosomes, what types of information could you learn about the mechanism of DNA replicati...
See AnswerQ: As described in Table 11.3, what is the difference
As described in Table 11.3, what is the difference between a rapidstop and a slow-stop mutant? What are different roles of the proteins that are defective in rapid-stop and slow-stop mutants? From Fi...
See AnswerQ: The technique of dideoxy sequencing of DNA is described in Chapter 21
The technique of dideoxy sequencing of DNA is described in Chapter 21. The technique relies on the use of dideoxyribonucleotides. A dideoxyribonucleotide has a hydrogen atom attached to the 3′ carbon...
See AnswerQ: At which level is incomplete dominance more likely to be observed—
At which level is incomplete dominance more likely to be observed—at the molecular/cellular level or at the organism level?
See AnswerQ: Another technique described in Chapter 21 is polymerase chain reaction (PCR
Another technique described in Chapter 21 is polymerase chain reaction (PCR) which is based on our understanding of DNA replication. In this method, a small amount of double-stranded template DNA is m...
See AnswerQ: A research group has sequenced the cDNA and genomic DNA for a
A research group has sequenced the cDNA and genomic DNA for a particular gene. The cDNA is derived from mRNA, so it does not contain introns. Here are the DNA sequences. cDNA: 5′&ac...
See AnswerQ: Chapter 21 describes a technique known as Northern blotting that is used
Chapter 21 describes a technique known as Northern blotting that is used to detect RNA transcribed from a particular gene. In this method, a specific RNA is detected using a short segment of cloned DN...
See AnswerQ: An electrophoretic mobility shift assay (EMSA) can be used to
An electrophoretic mobility shift assay (EMSA) can be used to study the binding of proteins to a segment of DNA. This method is described in Chapter 21. When a protein binds to a segment of DNA, it sl...
See AnswerQ: As described in Chapter 21 and in experimental question E3, an
As described in Chapter 21 and in experimental question E3, an electrophoretic mobility shift assay can be used to determine if a protein binds to DNA. This method can also determine if a protein bind...
See AnswerQ: The technique of DNase I footprinting is described in Chapter 21.
The technique of DNase I footprinting is described in Chapter 21. If a protein binds over a region of DNA, it will protect the DNA in that region from digestion by DNase I. To carry out a DNase I foot...
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