Questions from Genetics

Q: Explain the meaning of the name reverse transcriptase.

Explain the meaning of the name reverse transcriptase.

Q: What is an advantage of making a cDNA library rather than a

What is an advantage of making a cDNA library rather than a genomic library?

Q: What is horizontal gene transfer?

What is horizontal gene transfer?

Q: After four cycles of PCR, which type of PCR product predominates

After four cycles of PCR, which type of PCR product predominates? Explain why.

Q: What needs to happen so the reporter molecule can emit fluorescence that

What needs to happen so the reporter molecule can emit fluorescence that is not quenched?

Q: Describe three possible uses of site-directed mutagenesis.

Describe three possible uses of site-directed mutagenesis.

Q: How is the sgRNA different from certain components of the bacterial defense

How is the sgRNA different from certain components of the bacterial defense system described in Chapter 17?

Q: What is the purpose of using a secondary antibody?

What is the purpose of using a secondary antibody?

Q: What is the purpose of using CNBr in this experiment?

What is the purpose of using CNBr in this experiment?

Q: What is the difference between a gene knockout and a gene knockin

What is the difference between a gene knockout and a gene knockin?