Cell culture is a process in which the cells are isolated from the animals and plants and they are then grown in an artificial environment. These cells are obtained in two ways. One is that they are removed directly from the tissues and are segregated by enzymatic and mechanical means prior to their cultivation. The other way is to isolate them from the cell lines which are already been established. They are kept in an artificial medium which provides them with all the essential nutrients.
Moreover, they are also provided with growth factors, hormones, a constant supply of oxygen, and carbon dioxide. The temperature, pH, and the osmotic pressure of the medium in which the cells are present are also maintained. These cell cultures have proved them a very important tool as they are used in studying the physiology and biochemistry of cells. They are also employed when the large-scale manufacturing of the vaccines and therapeutic proteins is done.
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