Definition of Chromatin Immunoprecipitation



In the eukaryotic cells, the DNA and the proteins form a complex inside the nucleus which is termed as chromatin. The interaction between the DNA and proteins is quite essential for the normal functioning of the cells e.g., separation of chromosomes, gene transcription, DNA replication, etc. These interactions are studied by an advantageous technique which is known as chromatin immunoprecipitation.

 


This technique enables us to determine whether a certain protein binds to a particular gene sequence or not. The level of methylation of histone proteins associated with the certain region of gene promotor in the normal and the diseased tissue can also be compared. It becomes easy to understand the cellular processes when the targets of DNA binding proteins are identified.

 

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