Definition of Column Chromatography

Column Chromatography is a technique used to separate a mixture of substances. The substances that are to be separated (eluent) are introduced into the column that is made up of metal or glass. The column is formed by the close packing of an adsorbent. The eluent is then allowed to pass through this column.


The rate of the substance is dependent upon the affinity between the substance and the absorbent molecules. The stationary phase is a solid and the mobile phase is a liquid (liquid chromatography) or a gas (gas chromatography). it is used in the removal of impurities, isolation of metabolites from the biological fluid, etc.

View More Organic Chemistry Definitions