Definition of Complementary Dna

Complementary DNA is a double-stranded DNA that is formed as a result of a process known as reverse transcription. The template used is messenger RNA and the process is catalyzed by an enzyme which is named reverse transcriptase.


Complementary DNA plays a role where there is a need to clone eukaryotic genes in the prokaryotic cells. This cloning is done in order to express certain proteins. The retroviruses such as HIV-1 have the ability to naturally synthesize this DNA. The mRNA which forms this complementary DNA is devoid of introns (non-coding region of a gene).


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