Definition of Immunoprecipitation



Immunoprecipitation is a technique in which precipitation of the molecules such as proteins is done with the help of antibodies. The protein is an antigen and it is precipitated out of the solution by employing an antibody that binds to that specific protein. If the sample is composed of a number of proteins, then in situ hybridization is used in order to separate and concentrate the particular protein.

 


This is the most widely used technique that purifies and detects antigens. It also enables the researchers to evaluate the active status of the proteins, to determine the post-translational modifications in proteins, to estimate the molecular weight of the protein of interest, etc.

 

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