Definition of Isoelectric Focusing

Isoelectric focusing is a form of gel electrophoresis in which the proteins are migrated to such a point in the gel where the net charge is zero and their motion ceases and they become focused into sharp stationary bands. It is also known as electro focusing. This technique is used for separating different molecules present in a sample on the basis of their isoelectric points (pI).


The ampholyte solution is added to the immobilized pH gradient gel. These IPG gels are actually the acrylamide gel matrix that is polymerized with the pH gradient. This results in the formation of completely stable gradients. There is a continuous change in the ratio of the immobilines.

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