Definition of Phosphodiester Linkage

Phosphodiester linkage is a bond that is formed when two hydroxyl groups of the phosphate react with the hydroxyl groups present in the other molecules. This reaction leads to the formation of two ester bonds.


Example of Phosphodiester Linkage:

An example of such a bond is found in the sugar-phosphate backbone of DNA and RNA molecules. The two pentose sugar molecules are linked with the phosphate group by these covalent ester bonds. With the formation of one ester bond, a molecule of water is released. The 3’ carbon of one nucleotide is bonded to the 5’ carbon of the other nucleotide in order to form the strands of DNA and RNA.


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