Reverse Transcription Polymerase Chain Reaction is a technique which is responsible for detecting and quantifying the RNA which has been extracted from even a single cell. It is a very sensitive technique as it requires a minute quantity of the sample. On the contrary, there are two more techniques i.e., Northern blot and nuclease protection assays which are widely used but require large amounts of RNA.
One major problem that arises in RT-PCR is that the genomic DNA gets mixed up with RNA which leads to incorrect results. This reaction is catalyzed by an enzyme which is known as reverse transcriptase. It uses RNA as a template and forms complementary DNA which is not prone to degradation by RNase. The infections caused by RNA viruses i.e., HIV, HCV etc. are diagnosed and quantified by this technique.
How is an F′ factor different from an F factor?
Meiotic nondisjunction is much more likely than mitotic nondisjunction. Based on
In fruit flies, curved wings are recessive to straight wings,
What is an advantage and a disadvantage of this repair system?
Explain the type of speciation (allopatric, parapatric, or sympatric
The raw material for evolution is random mutation. Discuss whether or
With regard to the timing of conjugation, explain why the recipient
A woman who is heterozygous, Bb, has brown eyes;
A recessive allele in mice results in an unusally long neck.
Why is an antibody used in this experiment? From
