Q: What phase of PCR (exponential, linear, or stationary)
What phase of PCR (exponential, linear, or stationary) is analyzed to quantitate the amount of DNA or RNA in a sample? Explain why this phase is chosen.
See AnswerQ: DNA sequencing can help us to identify mutations within genes. The
DNA sequencing can help us to identify mutations within genes. The following data are derived from an experiment in which a normal gene and a mutant gene have been sequenced: Locate and d...
See AnswerQ: What is the advantage of genetic recombination, which is depicted in
What is the advantage of genetic recombination, which is depicted in part (b)? From Figure 20.1:
See AnswerQ: Table 21.3 describes the cleavage sites of five different restriction
Table 21.3 describes the cleavage sites of five different restriction enzymes. After these restriction enzymes have cleaved the DNA, four of them produce sticky ends that can hydrogen bond with comple...
See AnswerQ: Describe the important features of cloning vectors. Explain the purpose of
Describe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.
See AnswerQ: How does gene cloning produce many copies of a gene?
How does gene cloning produce many copies of a gene?
See AnswerQ: In your own words, describe the series of steps necessary to
In your own words, describe the series of steps necessary to clone a gene.
See AnswerQ: What is a recombinant vector? How is a recombinant vector constructed
What is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in a method of identifying recombinant vectors that contain segments of chromosomal DNA.
See AnswerQ: What is a DNA library? Do you think this name is
What is a DNA library? Do you think this name is appropriate?
See AnswerQ: Some vectors used in cloning experiments contain bacterial promoters that are adjacent
Some vectors used in cloning experiments contain bacterial promoters that are adjacent to unique cloning sites. This makes it possible to insert a gene sequence next to the bacterial promoter and expr...
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