Q: Tumor-suppressor genes are normal human genes that prevent uncontrollable cell
Tumor-suppressor genes are normal human genes that prevent uncontrollable cell growth. Starting with a normal laboratory human cell line, describe how you could use transposon tagging to identify tumo...
See AnswerQ: Gerald Rubin and Allan Spradling devised a method of introducing a transposon
Gerald Rubin and Allan Spradling devised a method of introducing a transposon into Drosophila. This approach has been important for the transposon tagging of many Drosophila genes. The researchers beg...
See AnswerQ: Richard Boyce and Paul Howard-Flanders conducted an experiment that provided
Richard Boyce and Paul Howard-Flanders conducted an experiment that provided biochemical evidence that thymine dimers are removed from DNA by a DNA repair system. In their studies, bacterial DNA was r...
See AnswerQ: A researcher identified a mutation in PR of phage λ that causes
A researcher identified a mutation in PR of phage λ that causes its transcription rate to be increased 10-fold. Do you think this mutation would favor the lytic or lysogenic cycle? Explain your answer...
See AnswerQ: Experimentally, when an E. coli bacterium already has a λ
Experimentally, when an E. coli bacterium already has a λ prophage integrated into its chromosome, another λ phage cannot usually infect the cell and establish the lysogenic or lytic cycle. Based on y...
See AnswerQ: A bacterium is exposed to a drug that inhibits the N protein
A bacterium is exposed to a drug that inhibits the N protein. What would you expect to happen if the bacterium was later infected by phage λ? Would phage λ follow the lytic cycle, the lysogenic cycle,...
See AnswerQ: What is the functional significance of sticky ends in a cloning experiment
What is the functional significance of sticky ends in a cloning experiment? What type of bonding makes the ends sticky?
See AnswerQ: Starting with a sample of RNA that contains the mRNA for the
Starting with a sample of RNA that contains the mRNA for the β-globin gene, explain how you could create many copies of the β-globin cDNA using reverse transcriptase PCR.
See AnswerQ: What type of probe is used for real-time PCR?
What type of probe is used for real-time PCR? Explain how the level of fluorescence correlates with the level of PCR product.
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