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Question: In the CRISPR-Cas system, does the


In the CRISPR-Cas system, does the tracrRNA act as a scaffold, guide, ribozyme, blocker, decoy, and/or alterer of protein function or stability?



> What is diauxic growth? Explain the roles of cAMP and CAP in this process.

> What is the difference between a constitutive gene and a regulated gene?

> Explain the role of HIV protease during the process of HIV maturation.

> How does an HIV particle acquire its envelope?

> Describe the role of the Gag polyprotein during the assembly of HIV components at the host-cell plasma membrane.

> Compare and contrast the roles of fully spliced, incompletely spliced, and unspliced HIV RNA. Which type is needed in the early stages of HIV proliferation, and which is needed in later stages?

> Why is gap repair synthesis needed during HIV DNA integration?

> What is the role of the Vpr protein during the process of HIV DNA integration?

> Explain why histone eviction is needed for the elongation phase of transcription.

> Describe how HIV DNA is integrated into a chromosome of the host cell.

> Explain the role of RNase H (a component of reverse transcriptase) during the synthesis of HIV DNA.

> What structural features are common to all viruses? Which features are found only in certain types of viruses?

> Why is a host-cell tRNA needed for reverse transcription?

> Describe the process of reverse transcription of HIV RNA.

> Figure 18.11 shows a genetic switch that controls the choice between the lytic and lysogenic cycles of phage λ. What is a genetic switch? Compare the roles of a genetic switch and a simple operator site (like the one found in the

> In your own words, explain why it is necessary for the cI gene to have two promoters. What would happen if it had only PRE?

> How do the λ repressor and the cro protein affect the transcription from PR and PRM? Explain where these proteins are binding to cause their effects.

> With regard to promoting the lytic or lysogenic cycle, what would happen if the following genes were missing from the λ genome? A. cro B. cI C. cII D. int E. cII and cro

> Describe the role that integrase plays during the insertion of λ DNA into the host chromosome.

> Why is an NFR needed at the core promoter for transcription to occur?

> What key features distinguish the lytic from the lysogenic cycle?

> What is a prophage, a provirus, and an episome? What is their common role in a viral reproductive cycle?

> What is the difference between a temperate phage versus a virulent phage?

> Discuss why viruses are considered nonliving.

> Describe the structure of SRP in eukaryotes, and outline its role in targeting proteins to the ER membrane.

> Together with a specific set of proteins, snoRNAs direct the methylation or pseudouridylation of rRNAs. Does the snoRNA function as a scaffold, guide, ribozyme, blocker, decoy, and/or alterer of protein function or stability?

> What is the difference between an miRNA and an siRNA. How do these ncRNAs affect mRNAs?

> With regard to RNAi, what are three possible sources for doublestranded RNA?

> What is the phenomenon of RNA interference (RNAi)? During RNAi, explain how the double-stranded RNA is processed and how it leads to the silencing of a complementary mRNA.

> Explain how HOTAIR plays a role in the transcriptional regulation of particular genes. 

> Describe two different ways that histone modifications may alter chromatin structure.

> What is meant by the term RNA world? Describe observations and evidence that support this hypothesized period of life on Earth. From the perspective of living cells, what are the advantages of having had the RNA world be superseded by a DNA/RNA/ protein

> Describe how the binding of iron regulatory protein to an IRE affects the mRNAs for ferritin and the transferrin receptor. How does iron (Fe3+) influence this process?

> What is a CpG island? Where would you expect one to be located? How does the methylation of CpG islands affect gene expression?

> Let’s suppose that a vertebrate organism carries a mutation that causes some cells that normally differentiate into nerve cells to differentiate into muscle cells. A molecular analysis reveals that this mutation is in a gene that encodes a DNA methyltran

> What is DNA methylation? When we say that DNA methylation is heritable, what do we mean? How is it passed from a mother to a daughter cell?

> What is an insulator? Describe two different ways that insulators may exert their effects.

> Histones are thought to be displaced as RNA polymerase is transcribing a gene. What would be the potentially harmful consequences if histones were not put back onto a gene after RNA polymerase had passed?

> What is a nucleosome-free region? Where are such regions typically found in a genome? How are nucleosome-free regions thought to be functionally important?

> What is meant by the term histone code? With regard to gene regulation, what is the proposed role of the histone code?

> An ncRNA may have the following functions: scaffold, guide, alterer of protein function or stability, ribozyme, blocker, and/or decoy. Which of those functions is/are mediated by each of the ncRNAs listed next? (Note: A single ncRNA may have more than on

> What are the two ways in which piRNAs and PIWI proteins prevent the movement of transposable elements?

> Explain how the acetylation of core histones may loosen chromatin packing.

> What is a histone variant?

> Explain how the VIN3/PRC2 complex specifically binds to the FLC gene.  

> Explain how the miR-200 family of miRNAs behave as tumorsuppressor genes. What happens when their expression is blocked or decreased?

> List five types of cancer in which ncRNAs can be involved.

> Outline the steps that occur when piRISCs silence transposable elements by repressing transcription and by directly inhibiting TE RNAs. What is the role of piRNAs in this process?

> What are the roles of Cas1, Cas2, and Cas9 proteins in bacterial genome defense?

> Compare and contrast the roles of crRNA and tracrRNA in the defense process against bacteriophages provided by the CRISPRCas system.

> Look at Figure 17.6 and predict what would happen if the SRP RNA was unable to stimulate the GTPase activities of the GTPbinding proteins within SRP and the SRP receptor. From Figure 17.6: Ribosome 5' 3' ER signal sequence NH, As a polypeptide is b

> Which component of the CRISPR-Cas system directly recognizes the bacteriophage DNA?

> List and briefly describe four types of molecules that can bind to an ncRNA.

> In general, explain how epigenetic modifications are an important mechanism for developmental changes that lead to specialized body parts and cell types. How do the trithorax and polycomb group complexes participate in this process?

> Following X-chromosome inactivation, most of the genes on the inactivated X chromosome are silenced. Explain how. Name one gene that is not silenced.

> Outline the molecular steps in the process of X-chromosome inactivation (XCI). Which step plays a key role in choosing which of the X chromosomes will remain active and which will be inactivated?

> Let’s suppose a mutation removes the ICR next to the Igf2 gene. If this mutation is inherited from the mother, will the Igf2 gene (from the mother) be silenced or expressed? Explain.

> Explain how DNA methylation and the formation of a DNA loop control the expression of the Igf2 gene in mammals. How is this gene imprinted so that only the paternal copy is expressed in offspring?

> What is the key difference between cis- and trans-epigenetic mechanisms for maintaining an epigenetic modification? In Chapter 5, we considered genomic imprinting of the Igf2 gene, in which offspring express the copy of the gene they inherit from their f

> Explain how epigenetic changes may be targeted to specific genes.

> List and briefly describe five types of molecular mechanisms that may underlie epigenetic gene regulation.

> If a winter-annual strain of Arabidopsis is grown in a greenhouse and not exposed to cold temperatures, its ability to flower is inhibited. Which gene is responsible for this inhibition?

> Why is GTP necessary for this process?

> Is paramutation a cis- or a trans-epigenetic mechanism?

> How can environmental agents that do not cause gene mutations contribute to cancer? Would these epigenetic changes be passed to offspring?

> Using coat color in mice and the development of female honeybees as examples, explain how dietary factors can cause epigenetic modifications, leading to phenotypic effects.

> With regard to development, what would the dire consequences be if polycomb group complexes did not function properly?

> Describe the molecular steps by which polycomb group complexes cause epigenetic gene silencing.

> What are the contrasting roles of trithorax and polycomb group complexes during development in animals and plants?

> Define epigenetics. Are all epigenetic changes passed from parent to offspring? Explain.

> Let’s suppose a mutation in the glucocorticoid receptor does not prevent the binding of the glucocorticoid hormone to the protein but prevents the ability of the receptor to activate transcription. Make a list of all the possible defects that may explain

> Describe the steps that need to occur for the glucocorticoid receptor to bind to a GRE.

> The binding of a small effector molecule, protein-protein interactions, and covalent modifications are three common ways to modulate the activities of transcription factors. Which of these three mechanisms are used by steroid receptors and by the CREB pr

> Which type of snoRNA causes an rRNA to be methylated?

> What is a clade?

> Transcription factors usually contain one or more motifs that play key roles in their function. What is the function of the following motifs? A. Helix-turn-helix B. Zinc finger C. Leucine zipper

> Is each of the following statements true or false? A. An enhancer is a type of regulatory element. B. A core promoter is a type of regulatory element. C. Regulatory transcription factors bind to regulatory elements. D. An enhancer may cause the down

> What are the functions of transcriptional activator proteins and repressor proteins? Explain how they work at the molecular level.

> What is meant by the term transcription factor modulation? List three general ways this can occur.

> Discuss the structure and function of regulatory elements. Where are they located relative to the core promoter?

> Briefly describe three ways that ATP-dependent chromatin-remodeling complexes may change chromatin structure.

> The gene that encodes the enzyme called tyrosine hydroxylase is known to be activated by the CREB protein. Tyrosine hydroxylase is expressed in nerve cells and is involved in the synthesis of catecholamine, a neurotransmitter. The exposure of cells to ad

> The DNA-binding domain of each CREB protein subunit recognizes the sequence 5′–TGACGTCA–3′. Due to random chance, how often would you expect this sequence to occur in the human genome, which contains approximately 3 billion base pairs? Actually, only a f

> An enhancer, located upstream from a gene, has the following sequence: 5′–GTAG–3′ 3′–CATC–5′ This enhancer is orientation-independent. Which of the following sequences also works as an enhancer? A. 5′–CTAC–3′ 3′–GATG–5′ B. 5′–GATG–3′ 3′–CTAC–5′ C.

> Transcription factors such as the glucocorticoid receptor and the CREB protein form homodimers and activate transcription. Other transcription factors form heterodimers. For example, a transcription factor known as myogenic bHLH forms a heterodimer with

> Explain why RISC binds to a specific mRNA. What type of bonding occurs?

> The glucocorticoid receptor and the CREB protein are two examples of transcriptional activators. These proteins bind to response elements and activate transcription. (Note: The answers to this question are not directly described in this chapter. You have

> A particular drug inhibits the protein kinase that is responsible for phosphorylating the CREB protein. How would this drug affect the following events? A. The ability of the CREB protein to bind to CREs B. The ability of extracellular hormones to enha

> Explain how phosphorylation affects the function of the CREB protein.

> Discuss the common points of control in eukaryotic gene regulation.

> If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not alter the conformation of the repressor protein, how would the expression of the lac operon be affected?

> In the lac operon, how would gene expression be affected if each one of the following segments was missing? A. lac operon promoter B. Operator site C. lacA gene

> What is enzyme adaptation? From a genetic point of view, how does it occur?

> Some mutations have a cis-effect, whereas others have a transeffect. Explain the molecular differences between cis- and transmutations. Which type of mutation (cis or trans) can be complemented in a merozygote experiment?

> An operon is repressible—a small effector molecule turns off its transcription. Which combination(s) of small effector molecule and regulatory protein could be involved in this process? A. An inducer plus a repressor B. A corepressor plus a repressor

> Transcriptional regulation often involves a regulatory protein that binds to a segment of DNA and a small effector molecule that binds to the regulatory protein. Do each of the following terms apply to a regulatory protein, a segment of DNA, or a small e

> What types of molecules can bind to a non-coding RNA?

> If a gene is repressible and under positive control, what kind of effector molecule and regulatory protein are involved in its regulation? Explain how the binding of the effector molecule affects the regulatory protein.

> Transcriptional repressor proteins (e.g., lac repressor), antisense RNA, and feedback inhibition are three different mechanisms that turn off the expression of genes and gene products. Which of these three mechanisms will be most effective in each of the

> How are the actions of lac repressor and trp repressor similar and how are they different with regard to their binding to operator sites, their effects on transcription, and the influences of small effector molecules?

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